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CEREBROVASCULAR REACTIVITY ASSESSMENT WITH SYSTEMIC USE OF CARBONIC ANHYDRASE INHIBITOR FOR RATS: CAPABILITIES OF IMAGING PHOTOPLETHYSMOGRAPHY

Oleg V. Mamontov, Pavlov First Saint Petersburg State Medical University, Saint Petersburg, Russia; Almazov National Medical Research Centre, Saint Petersburg, Russia
Alexey Y. Sokolov, Valdman Institute of Pharmacology, Pavlov First Saint Petersburg State Medical University, Saint Petersburg, Russia; Pavlov Institute of Physiology, RAS, Saint Petersburg, Russia
Maxim A. Volynsky, ITMO University, Saint Petersburg, Russia
Anastasija V. Osipchuk, Valdman Institute of Pharmacology, Pavlov First Saint Petersburg State Medical University, Saint Petersburg, Russia
Alexei A. Kamshilin, ITMO University, Saint Petersburg, Russia

Abstract

Assessment of the reserve of cerebral blood flow in patients with cerebrovascular diseases is extremely important in terms of making prognosis and determining treatment tactics. In addition, when performing reconstructive interventions on the brain vessels, monitoring of the blood flow changes is necessary for urgent intraoperative control of the revascularization outcome. However, there is a lack of an easy-to-use, contactless method capable for assessing the functional reserve of the cortical vasculature.

To demonstrate feasibility to exploit green-light imaging photoplethysmography (PPG) as a reliable contactless method allowing quick estimation of cerebrovascular functional reserve in conditions of the blood-flow increase due to provoked acidosis caused by an administration of the carbonic anhydrase inhibitor in animal model of craniosurgical intervention.

We used custom-made imaging PPG system to visualize intracranial vessels through a cranial window without violating the integrity of the cranial cavity. Experiments were carried out with anesthetized Wistar rats (n=16). Spatial distribution of the amplitude of the pulsatile component (APC) of the PPG waveform was found to be highly inhomogeneous and significantly increased after dorzolamide-stimulated acidosis for all animals under the study. Dynamics of the APC was recorded concurrently with physiological indices of external respiration, systemic blood pressure, and electrocardiogram. A significant increase in APC by 35±19% (p

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Maxim A. Volynsky
ITMO University
Russia

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