Identification and Analysis of Key Parameters for the Ossification of Cells on Composites Gellan Gum Hydrogels Functionalized by Ca/Mg carbonates.
A.A. Abalymov, L. Van der Meeren, A.G. Skirtach, B.V. Parakhonskiy
Saratov State University, Saratov, 410012 Russia
Ghent University, Ghent, 9000, Belgium
Abstract
Developing of biomaterials is a very difficult process that requires to have knowledge in biology, physics, and chemistry. Hydrogels are water-rich polymers wide use for bone reconstruction, but often, it has very low adhesion and ossification properties. Fortunately, сomposite hydrogels materials have complex architectures and tunable mechanical properties emerged as a powerful tool to control cell adhesion and proliferation for tissue engineering.
In current work, we have designed new osteoinductive gellan gum hydrogels by a thermal annealing approach and consequently functionalized them with Ca/Mg carbonate submicron particles. Determination of key parameters, which influence a successful hydroxyapatite generation was done via the principal component analysis of 18 parameters (Young’s modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production and cells area) obtained by characterizing such composite hydrogel.
The work was supported by the Russian Science Foundation (project No. 19-73-10123).
Speaker
Anatolii Abalymov
1. Saratov State University, Saratov, 410012 Russia. 2. Ghent University, Ghent, 9000, Belgium
Belgium
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