Biomedical applications of nonlinear optical microimaging techniques

Abstract

Abstract:
Optical microscopy imaging technology is a research hotspot in the field of biomedical photonics. In the past two decades, optical microscopy imaging technology has developed very rapidly, constantly breaking through the traditional limits, and increasingly used in the research on subcellular, cellular, tissue and in vivo imaging research，providing a unique perspective for real-time dynamic observation of life systems and solving many key scientific issues in the field of "biomedical photonics".
Our research group has carried out many years of basic application research of nonlinear optical microscopy imaging technology in biomedicine, covering Two-photon Excitation Fluorescence(TPF), Second-Harmonic Generation(SHG), and Stimulated Raman Scattering(SRS) at different levels of molecules, cells, tissues, and living bodies. These optical imaging technologies provide powerful technical support for the monitoring of the microenvironment during tumor treatment. With the help of mature optical microscopic imaging technology, the clinical progress of qualitative and quantitative analysis of the microenvironment during tumor treatment can be promoted.
Tumor microenvironment refers to the internal and external environment in which tumors occur, grow and metastasize. The relationship between tumor microenvironment and tumor is called "seed and soil". In the process of tumor diagnosis and treatment, tumor microenvironment plays a vital role. Therefore, imaging monitoring of the tumor microenvironment has become an assessment method in the process of tumor treatment.
This report mainly introduces the research work of non-linear optical microscopy imaging technology in tumor microenvironment imaging carried out by our team recently, including tumor metastasis path, intracellular calcium ion regulation, etc.
REFERENCES:
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2. Binglin Shen, Shiqi Wang, Ganapathi Bharathi, Yangping Li, Fangrui Lin, Rui Hu, Liwei Liu, Junle Qu, Rapid and Targeted Photoactivation of Ca2+ Channels Mediated by Squaraine To Regulate Intracellular and Intercellular Signaling Processes, Anal. Chem., 8497-8505, 2020.
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Speaker

Liwei Liu
Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education & Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University
China

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