Tissue optical clearing: from in vitro to in vivo
Dan ZHU1,2
1 Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, China
2 MoE Key Laboratory for Biomedical Photonics, Huazhong University of Science and Technology, China
Abstract
Biomedical photonics is currently one of the fastest growing fields of life sciences, which allows structural and functional analysis of tissues with high resolution and contrast unattainable by any other method [1-3]. However, the high scattering of turbid biological tissues limits the penetration of light, leading to strongly decreased imaging resolution and contrast as light propagates deeper into the tissue [4, 5]. Fortunately, novel tissue optical clearing technique could reduce the scattering of tissue and make it transparent for higher optical imaging quality [6-9]. This presentation will introduce the recently developed in vitro optical clearing methods for whole organs imaging, including FDISCO and MACS [10, 11]. And then I will demonstrate in vivo skull/skin optical clearing window for imaging structural and functional of cutaneous / cortical vascular and cells, also manipulating cortical vasculature [12-14].
Speaker
Dan ZHU
Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology
China
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