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Study of skin dehydration in the course of grafted tumor development using spectral refractometry, NIR and THz spectroscopy

Polina DYACHENKO (TIMOSHINA)1,2, Ekaterina LAZAREVA1,2, Maxim NAZAROV3, Alla BUCHARSKAYA4, Valery TUCHIN1,2,5 and Alexander SHKURINOV6

1 Saratov State University, Russia
2Tomsk State University, Russia
3NRC «Kurchatov Institute», Russia
4Saratov State Medical University, Russia
5Institute of Precision Mechanics and Control, Russian Academy of Sciences, Saratov, Russia
6 Lomonosov Moscow State University, Russia


In this work, experimental studies of the spectral characteristics of the skin of laboratory animals with an inoculated tumor when probed by an optical clearing agent (100% glycerol solution). The reflection spectra were recorded in the spectral range 900–2000 nm using a USB4000-Vis-NIR spectrometer (Ocean Optics, USA) and a QR400-7-Vis / NIR fiber probe (Ocean Optics, USA). The tumor was inoculated by subcutaneous injection of 0.5 ml of a 25% tumor cell suspension in the Hanks solution of PC1 alveolar liver cancer strain into the scapula area. NIR Spectroscopy measurements were taken from the skin area directly above the tumor formation and nearby (control area) without neoplasm. For the optical clearing study, a 99.3% glycerol solution was poured into a special cuvette in an amount of 1 ml, which openly touched the skin to ensure complete contact of the OCA with the skin surface for 30 min. After removal of the solution, reflection spectra were recorded from the studied area of the skin. Next, studies of the solution collected after 30 min of exposure to the skin were carried out using refractometry in the visible/NIR and THz spectroscopy. The refractive index of modified glycerol solutions was measured using an Abbe DR-M2 / 1550 multiwave refractometer (Atago, Japan). To select the wavelengths, interference filters were used for 480, 486, 546, 589, 644, 656, 680, 800, 930, 1100, 1300, and 1550 nm.
In both groups, an increase in the concentration of water in the skin of animals under the influence of OCA was recorded.

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Saratov State University, Russia; Tomsk State University, Russia


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