Multiparameter optical diagnostics of microcirculatory-tissue systems: methods and technical means
Andrey Dunaev, Research and Development Center of Biomedical Photonics, Orel State University named after I.S. Turgenev, Russia
Abstract
In the last decade, there has been a steady increase in the interest of researchers in the problems of non-invasive research of microcirculatory-tissue systems (MTS). This is due to the essential role of blood microcirculation in the pathogenesis of various diseases (in particular, diseases of the rheumatological and endocrinological profiles). A modern trend in the development of optical biomedical diagnostics is a multiparametric approach when various optical (and sometimes additionally other) research methods are combined in one diagnostic technology. This allows one to obtain highly efficient diagnostic tools for rheumatology, endocrinology, surgery, oncology, neurology, and other areas of medicine, where it is necessary to determine the parameters of the perfusion-metabolic status of tissues.
The aim of this work was to scientifically substantiate and develop a methodology for multiparametric optical non-invasive diagnostics (mOND) to assess the functional state of the microcirculatory-tissue systems of the human body with the development of metrological support of methods and technical means that contribute to improving the quality of optical non-invasive diagnostics and its wider implementation in clinical practice.
To solve the research problems, a systematic approach was used to develop a methodology for the synthesis of mOND for assessing the functional state of MTS in various diseases. A method for assessing angiospastic and microcirculatory [1] disorders in rheumatic diseases and a method for assessing microcirculatory-metabolic disorders in MTS of the lower extremities in diabetes mellitus [2] were developed. Also in this work, methods were developed for assessing the state of the MTS of the human body under various conditions, such as sports and physiological stresses [3], the provision of physiotherapeutic effects, during minimally invasive surgical interventions [4]. The developed methods are based on the combined application of several widely used methods of optical non-invasive diagnostics, such as laser Doppler flowmetry, diffuse reflection spectroscopy, fluorescence spectroscopy, etc.
Approbation of the developed methods was carried out on the basis of 3 departments of the Orel Regional Clinical Hospital. The reliability of the results obtained has been confirmed by numerous clinical observations of more than 200 patients.
The results of the work can be extended to other areas of medicine, for example, in the direction of improving methods of optical biopsy in minimally invasive surgery, rheumatology, endocrinology, otolaryngology, dermatology, neurology and other areas of medicine. The introduction of mOND into wearable devices (fitness bracelets, gadgets) for long-term monitoring (daily or during sleep, tracking of circadian biorhythms) and monitoring in vivo (and not only in a hospital) also has great diagnostic potential.
The study was supported by the Russian Science Foundation (the project №18-15-00201), Russian Foundation for Basic Research (the project 18-02-00669) and by the grant of the Russian Federation Government (the project №075-15-2019-1877).
[1] A.I. Zherebtsova, V.V. Dremin, I.N. Makovik, E.A. Zherebtsov, A.V. Dunaev, A.Goltsov, S.G. Sokolovski, E.U. Rafailov Multimodal Optical Diagnostics of the Microhaemodynamics in Upper and Lower Limbs, Front. Physiol., 10, 416, 2019.
[2] V. Dremin, E. Zherebtsov, V. Sidorov, A. Krupatkin, I. Makovik, A. Zherebtsova, E. Zharkikh, E. Potapova, A. Dunaev, A. Doronin, A. Bykov, I. Rafailov, K. Litvinova, S. Sokolovski, E. Rafailov Multimodal optical measurement for study of lower limb tissue viability in patients with diabetes mellitus, Journal of Biomedical Optics, 22(8), 085003, 2017.
[3] A.V. Dunaev, V.V. Sidorov, A.I. Krupatkin, I.E. Rafailov, S.G. Palmer, N.A. Stewart, S.G. Sokolovski, E.U. Rafailov Investigating tissue respiration and skin microhaemocirculation under adaptive changes and the synchronization of blood flow and oxygen saturation rhythms, Physiological Measurement, 35(4):607, 2014.
[4] V. Dremin, E. Potapova, E. Zherebtsov, K. Kandurova, V. Shupletsov, A. Alekseyev, A. Mamoshin, A. Dunaev Optical percutaneous needle biopsy of the liver: a pilot animal and clinical study, Scientific Reports, 10, 14200, 2020.
Speaker
Andrey Dunaev
Research and Development Center of Biomedical Photonics, Orel State University named after I.S. Turgenev
Russia
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