FIBER OPTIC SOLUTIONS FOR BIOMEDICINE

Abstract

One of the streams in advanced diagnostic technologies is non-invasive spectroscopic
investigation or “spectral histopathology” as a novel alternative for rapid cancer diagnostics
and label-free cancer specification. Fiber optics enable faster and more convenient way to
study different biological tissues than standard techniques which require destructive sample
preparation (e.g. histopathology, chemical analysis). Moving on this direction, we developed
and applied various fiber optic probes for key spectroscopy methods such as Raman
scattering, Mid IR-absorption, Diffuse NIR-reflection, and auto-fluorescence – to compare
them and select the best combination for a real-time detection of malignant tissue in pre-
clinical and clinical environment. All four spectroscopic methods have been tested for cancer
diagnostics on biopsies of normal and cancer tissues (abdominal, oral and brain), ex-vivo
samples and bioliquids. Obtained spectral data were evaluated by multivariate discrimination
analysis to enable clear separation of malignant and normal tissues. Benefits of combination
of several spectroscopic modalities and data fusion is presented for the better sensitivity,
specificity and accuracy. The best synergetic effect was observed of combining Mid IR-
absorption and fluorescence spectroscopy, (98% Sensitivity vs 63% or 88% for fluorescence
or Mid IR-absorption correspondingly). Based on obtained results, several fiber optic probes
combining several spectroscopic modalities implemented within the same single probe were
designed, assembled and evaluated.

Speaker

Olga Bibikova
art photonics GmbH
Germany

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