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Advanced spectral analysis of dentinal materials for dentistry during their manufacture

P.E. Timchenko 1, E.V. Timchenko 1, Volova L.Т. 2, O.O. Frolov 1, Zybin М.А. 2
1Samara National Research University, 34, Moskovskoye shosse, Samara, 443086, Russia;
2Samara State Medical University, Institute of Experimental Medicine and Biotechnology, Samara, st. Gagarin, 20

Abstract

Lasers are widely used to analyze the component composition of biomaterials. Intraosseous implants are increasingly used in wide dental practice in the treatment of patients with defects in the dentition of various localization. Since more and more often patients with partial and complete adentia refuse to use removable prostheses, work is actively underway to improve materials for replacing bone defects and stimulating its growth. These factors contribute to the further search for ways to expand the indications for dental implantation surgery. One of the possible materials for bone tissue atrophy in dentistry can be used human dentin. Nowadays, optical methods are increasingly used to evaluate biomaterials. Spectral assessment of the quality of manufactured biomaterials at any stage of production can be carried out in real time using the Raman spectroscopy method.
As objects of research, 21 dentin materials of adult human teeth were examined. All samples were divided into three groups according to the degree of processing: before processing (mineralized dentin), intermediate stage (dentin during demineralization), final product (dentin at the end of the demineralization process). Samples were made using Lioplast technology (TU-9398-001-01963143-2004).
Raman spectroscopy (RS) was chosen as the main research method. The method of modeling the spectral contour to increase the information content of the spectra was implemented in the MagicPlotPro 2.9.1 software environment, where the spectrum was decomposed into lines described by the Gaussian function. Further chemometric analysis using linear discriminant analysis (LDA) is implemented in the SPSS Statistics 23 software environment.
To study the influence of the studied criteria in the production of implants, the intensities of the CR lines 1446, 1739 (lipids & fatty acid), 851 and 935 cm-1 (proline & hydroxyproline), 1000 and 1026 cm-1 (phenylalanine), 812 and 1416 cm-1 (DNA), 1238, 1550 cm-1 (amide II, amide III), as well as hydroxyapatite lines: 960, 1047, 1069, 1093 cm-1.
With the help of spectral analysis, it was shown that the intensity of the line at 960 cm-1, corresponding to the mineral component of the studied materials, completely disappears in the demineralized samples. A decrease in the intensity of the line by 1739 cm-1 (phospholipids) in the process of demineralization was also found.
Advanced spectral analysis using spectral line separation and chemometric methods reveals the main differences in the composition of samples at different stages of bioimplant production. The investigated differences in the lines of Raman scattering can be used in the future for the rapid assessment of the quality of dentin materials during production.

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Frolov Oleg
Samara unversity
Russia

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