The Determination of Polarized Fluorescence Parameters of FAD in Water-Methanol Solutions
M.K. Krasnopevtceva, V.P. Belik, I.V. Semenova, A.G. Smolin,and O.S. Vasyutinskii
Ioffe Institute, 26 Polytechnicheskaya ul., St. Petersburg, Russia
Abstract
Optical properties of the biological cofactor flavin adenine dinucleotide (FAD) in water-methanol solutions at 0%, 20%, 40%, 60% and 80% methanol concentration have been studied. Two fluorescence lifetimes, corresponding weighting coefficients, anisotropy, and rotational diffusion time were determined from experiment through analysis of the polarized fluorescence decay excited by picosecond laser pulses. The dependence of the fluorescence parameters on solution polarity and viscosity has been analyzed.
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Krasnopevtceva M.K.
Ioffe Institute
Russia
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