A potential of optical coherence tomography for the differentiation of intact brain tissue, human brain gliomas of different WHO grades and glioma model 101.8
Brain glioma is the most common type of tumor of the central nervous system. The key-factor for the successful treatment is its total resection. This task is not always feasible and uncompleted surgical resection often leads to the tumor recurrence. The existing methods of the intraoperative neurodiagnosis have various limitations. Optical coherence tomography (OCT) is one of the promising tools for the intraoperative diagnosis of brain tumors, which is able to improve the efficiency of glioma margins detection. However, a number of factors impact on the prospects of OCT, such as strong variability of structural and optical properties of brain tissues. In order to study this impact, the analysis of attenuation coefficient, coefficient based on effective refractive index, and their standard deviations obtained from OCT measurements of ex vivo human and rat brain tissue samples was performed. The set of samples comprised intact white and gray matter, as well as human brain gliomas of the World Health Organization (WHO) Grades I-IV and glioma model 101.8 in rats. The physically-reasonable properties of tissues were analyzed by means of a linear discriminant analysis and estimation of their dispersion in a four-dimensional principal component space.
The obtained results show the significant variability of healthy and tumorous tissues’ optical properties and the essential differences between rat and human glioblastoma samples. The growing heterogeneity of pathological brain tissues correlates with the enlarged dispersion of properties with increase of the glioma WHO Grades. The results allow for revealing of the benefits and weaknesses of OCT for the intraoperative diagnosis of brain tumors.
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A.M. Prokhorov General Physics Institute of the RAS
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