Amplification of SERS-based detection of some antibiotics using cyclodextrin modified silver nanoparticles
Natalia E. Markina, Andrey M. Zakharevich, Alexey V. Markin
Saratov State University, Saratov, Russia
Abstract
The work is dedicated to the use of cyclodextrins (CDs) to improve detection of some antibiotics using surface-enhanced Raman spectroscopy (SERS). The following antibiotics of different classes have been used: ampicillin (penicillin), sulfasalazine (sulfanilamide), levofloxacin (fluoroquinolone). CDs were used for (i) synthesis and stabilization of silver nanoparticles (SERS substrate), (ii) increase of SERS signal intensity (up to 10 times compared to silver nanoparticles without CD modification) due to improvement of interaction between analyte molecules and the substrate. SERS spectra of antibiotics were recorded at different pH values and for each analyte optimal registration conditions, corresponding to the maximum signal enhancement, were different. Modification of SERS substrate with CD molecules also reduces SERS signal of some background-forming components of body fluids. Finally, the analytes were detected in synthetic urine at concentrations which are suitable for therapeutic drug monitoring.
The work was supported by the Russian Science Foundation (project 21-73-00098).
Speaker
Natalia E. Markina
Saratov State University
Russia
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