NANOCOMPOSITES OF GOLD AND GALLIUM-DOPED CFO NANOPARTICLES FOR PHOTOTHERMAL THERAPY
S.E. Pshenichnikov1*, A.V. Motorzhina1, V.V. Malashchenko2, S. Jovanović3,4, L.S. Litvinova2, L.V. Panina1,5, V.V. Rodionova1, K.V. Levada1
1Research & Education Center "Smart Materials & Biomedical Applications", Immanuel Kant Baltic Federal University, Kaliningrad, Russia.
2Center for Immunology and Cellular Biotechnology, Immanuel Kant Baltic Federal University, Kaliningrad, Russia.
3Department of Physics, Vinča Institute of Nuclear Sciences – National Institute of the Republic of Serbia, University of Belgrade, Belgrade, Serbia.
4Advanced Materials Department, Jožef Stefan Institute, Ljubljana, Slovenia.
5National University of Science and Technology «MISIS», Moscow, Russia
*e-mail: SPshnikov@gmail.com
Abstract
Nanocomposites are multicomponent materials, which can combine a wide range of physical and structural characteristics. Each characteristic affects potential applicability and physical properties of a nanocomposite. The combinaton of different characteristics makes it possible to create multifunctional materials for solving specific biomedical problems.
In research we propose nanocomposites with gold nanoparticles surrounded by gallium-doped Co-ferrite (CFO_Ga) and zink-doped (CFO_Zn) nanoparticles and covered with arginine, as an innovative structure for using in photothermal therapy and magnetomechanics techniques.
Gold nanoparticles were used as biocompatible core with potential applicability in MRI and photothermal therapy. CFO_Ga/Zn doped magnetic properties are helpful for combined photo – and magnetotherapies. Nanocomposite was covered with arginine to decrease cytotoxicity. Structural characterization of the composite showed that the average size was 13.9 ± 3.8 nm and 5.2 ± 1.1 nm of gold and CFO nanoparticles, respectively. According to the results obtained with the cytotoxicity analysis, Au(arg): CFO_Zn/Ga composites induced doze-dependent cytotoxic effect on three human cell lines (PBMC, Jurkat, Huh7). The composite reduced the viability of PBMC and Huh7 at all three concentrations. At the same time, the viability of Jurkat cells was not suppressed by 10 μg/ml treatment. Microscopy visualization showed that Huh7 morphology did not change after 24h treatment with 100 μg/ml. Viability of Huh7 cell line was greatly inhibited after photothermal treatment, using 785 nm laser. During the photothermal experiments overheating of experimental cells in comparison with control group is more than 4 °C.
The reported study was funded by RSF grant number 21-72-20158.
Speaker
Stanislav Pshenichnikov
Immanuel Kant Baltic Federal University
Russia
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