Metabolic imaging as a tool for the analysis of regenerative potential and liver pathology
Vadim V. Elagin,1, Svetlana A. Rodimova,1,2, Nikolay V. Bobrov,1,3, Ilya D. Shchechkin,1,2, Dmitry P. Krylov,1,2, Dmitry S. Kozlov,1,2, Maria M. Karabut,1, Artem M. Mozherov,1, Vladimir E. Zagainov,1,4, Elena V. Zagaynova,1,2, Daria S. Kuznetsova,1,2
1 - Privolzhsky research medical university, Nizhny Novgorod, Russia
2 - Lobachevsky Nizhny Novgorod National Research State University, Nizhny Novgorod, Russia
3 - The Volga District Medical Centre of Federal Medical and Biological Agency, Nizhny Novgorod, Russia
4 - Nizhny Novgorod Regional Clinical Oncologic Dispensary, Nizhny Novgorod, Russia
Abstract
Despite modern advances in surgical technique and improvement of methods for preoperative assessment of liver function, now there is a high risk of developing postoperative liver failure associated with a difference in the level of regenerative potential of the liver of each patient. The main idea of the study is to determine new criteria for the regenerative potential of the liver and the prevention of undersized liver syndrome, which could be assessed intraoperatively. To date, we have studied the metabolic status of hepatocytes, the collagen content in the liver tissue of laboratory animals during normal regeneration, as well as in the presence of liver pathologies. In the study, we used a set of metabolic bioimaging methods that can be introduced into the clinic, since there is an instrumental implementation of multiphoton microscopy for intravital examination of organs and tissues of patients. An important result of our study was the identification of a significant correlation between the percentage of liver recovery parameter and the contribution of the phosphorylated form of NADPH before the induction of regeneration, as well as with an increase in the contribution of the bound form of NADH in the early stages of liver recovery. High values of these parameters can be considered as criteria for successful liver regeneration.
Speaker
Vadim Elagin
Privolzhsky Research Medical University
Russia
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