WPI hydrogels with prolonged antimicrobial effect for biomedical application
Valentina O. Plastun 1, Ekaterina S. Prikhozhdenko 1, Olga I. Gusliakova 1, Svetlana V. Raikova 2,3, Olga A. Sindeeva 4 and Oksana A. Mayorova 1,5
1 Saratov State University, Saratov, Russia;
2 Saratov State Medical University Saratov, Russia;
3 FSC Medical and Preventive Health Risk Management Technologies, Saratov, Russia
4 Skolkovo Institute of Science and Technology, Moscow, Russia;
5 Saratov State Vavilov Agrarian University, Saratov, Russia
Abstract
Infectious sequelae caused by surgery are a significant problem in modern medicine. Recently, new methods of local antimicrobial prophylaxis of postoperative sequelae have been actively developed. They allow high local concentrations of drugs to be achieved, increasing the antibiotic therapy’s effectiveness while reducing its side effects. We have developed and characterized antimicrobial hydrogels based on an inexpensive and biocompatible natural substance from the dairy industry. We used whey protein isolate as matrices for drug delivery. The technique for drug loading into this type of hydrogel is simple without any losses of the drug. The release of cefazolin from the pores of hydrogel structures directly depends on the amount of the loaded drug and occurs in a prolonged manner for three days. The amount of released antibiotic is high enough to suppress bacterial growth for 48 h for the hydrogel samples containing 5 mg/mL and 10 mg/mL of cefazolin. This effect is manifested both in the liquid medium and on the surface of the nutrient agar. The antimicrobial activity and the overall cytotoxic effect of cefazolin-containing WPI hydrogels is lower than that of free antibiotics.
The use of WPI hydrogels as carriers of antibiotics makes it possible to reduce their overall cytotoxicity against normal healthy cells. In this regard, it seems promising to use WPI hydrogels with a prolonged release of the drug as a material for coating implants and manufacturing other medical devices with antibacterial properties.
This research was funded by the Russian Science Foundation, grant number 21-75-10042.
Speaker
Valentina Plastun
Saratov State University
Russia
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