Quantitative phase imaging of transparent biological samples using off-axis digital holographic microscopy, TIE and SLIM
A.A. Zhikhoreva1, E.V. Beglova2, A.V. Belashov1, T.N. Belyaeva3, A.V. Salova3, E.S. Kornilova3, I.V. Semenova1, O.S. Vasyutinskii1
1 Ioffe Institute, 26, Polytekhnicheskaya, 194021 St. Petersburg, Russia
2 St. Petersburg Academic University, 194021 St. Petersburg, Russia
3 Institute of Cytology of RAS; 4, Tikhoretsky pr., 194064 St. Petersburg, Russia
Abstract
We present implementation of several quantitative phase imaging
techniques for evaluation of phase shift distributions in fixed cells.
Both coherent and partially coherent holographic techniques were used
for recording of QPI images: off-axis digital holographic microscopy,
transport of intensity equation (TIE) technique and spatial light
interference microscopy (SLIM). When applying TIE three commonly used
solvers were used and evaluated: FFT-based, discrete cosine transform
(DCT)-based and iterative DCT-based. Only insignificant difference was
found in performance of these TIE solvers, however the distortion-less
reconstruction of phase images was achieved only for isolated objects
located in the central part of the field of view. The DCT-based solvers
were shown to demonstrate higher reconstruction accuracy in the case of
inhomogeneous boundary conditions. Although off-axis digital holography
allowed for rapid and robust imaging of phase objects, coherent noise
often results in somewhat decrease in image quality. On the other hand
SLIM technique provided quantitative phase imaging with the highest
quality among the studied methods, however it requires recording of
several inline digital holograms corresponding to different phase shifts
in the reference (unscattered) wave.
Speaker
Anna Zhikhoreva
Ioffe Institute, 26, Polytekhnicheskaya, 194021 St. Petersburg, Russia
Russia
Discussion
Ask question
