Assessing the platelets degree of aggregation by light scattering under arterial hypertension and type 2 diabetes mellitus

Abstract

Type: poster

Title: Assessing the platelets degree of aggregation by light scattering under arterial hypertension and type 2 diabetes mellitus

Authors: D.A. Umerenkov, P.B. Ermolinskiy, A.E. Lugovtsov and A.V. Priezzhev
Speaker: D.A. Umerenkov
Faculty of Physics, Lomonosov Moscow State University, Moscow, Russia

WORKSHOPS: OPTICAL TECHNOLOGIES IN BIOPHYSICS

Platelets are critically important elements of the hemostatic system that prevent blood loss upon vessel wall disruption. Both conditions of bleedings or thrombosis might be an outcome of platelet function disorders; thus, the assessment of platelet function is vital for diagnostics and therapy. The aim of this work is to evaluate the parameters of platelet aggregation in patients with arterial hypertension and type 2 diabetes mellitus (T2DM).
Platelet aggregation parameters were assessed by light scattering. Light transmission platelet aggregometry (LTA) induced by adenosine diphosphate was performed for platelet-rich plasma (PRP) using both conventional light transmission and aggregate size monitoring method based on optical density fluctuations. Parameter values of the model were determined by means of parameter estimation techniques implemented in AGGR software.
Venous blood was taken from patients with arterial hypertension and diabetes mellitus. Sodium citrate 3.2% was used as an anticoagulant. To prepare PRP, whole blood was centrifuged in Eppendorf at 200g for 7 min. From each tube, 600 μl of supernatant (upper layer) was carefully taken. The PRP prepared for the study was poured in 300 µl into cuvettes for the aggregometer and placed in a thermostat at 37º for 3 min. The magnetic stirrer was rinsed in distilled water and placed with a magnet in the upper part of the cuvette, where it was held until the cuvette was transferred to the measuring cell. After 3 min, the magnetic stirrer was lowered to the bottom, the cuvette was placed in the measuring cell, and the start of measurements was initiated. After 10 seconds, as the measurement began, 15 μl of ADP at a concentration of 5 μM or 1 μM was added. Further, it was observed how platelet aggregatograms emerged.

The results show that the degree of aggregation in patients with arterial hypertension and T2DM is increased compared with people without these diseases. Based on the aggregatogram, it can be concluded that the degree of aggregation in the studied pathologies was 35±9% %, while the normal degree of aggregation was 25±3%
This work is supported by Russian Science Foundation Grant No. 20-45-08004.


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Speaker

Danila Umerenkov
Faculty of Physics, Lomonosov Moscow State University, Moscow, Russia
Russia

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