Fluorescent methods in the studies of retention and penetration of nanoparticles and polymers into the biological tissues
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Abstract
Advances in the imaging and optical methods have revolutionised many biological and biomedical applications. One of these applications is the evaluation of retention of various nano-objects and macromolecules on biological surfaces and their potential penetration deeply into these tissues. There are many areas where these methods will be applicable, for example, drug delivery, dental care, agriculture, cosmetics and other consumer products. This lecture will describe our recent developments of fluorescent imaging methods with nanocarriers, polymers and formulations for the following applications:
(1) Transmucosal and topical drug delivery: Retention of nanoparticles and polymers was evaluated on the ocular [1], urinary bladder [2], intestinal [3], and nasal tissues [4]. Penetration of fluorescently labelled nanoparticles through the ocular membranes [5], stomach mucus [6] and skin [7] were also evaluated.
(2) Rainfastness, which is defined as the ability of agrochemical formulations to withstand rain and retain on plant surfaces, is an important property that plays a major role in agriculture. We have developed the fluorescent methodology to evaluate rainfastness of several model polymer-containing formulations and established the factors affecting retention of the formulations [8, 9].
(3) Evaluation of toothpastes: Modern toothpaste formulations are very complex and contain a large range of active ingredients to provide a thorough cleaning of the mouth without damaging the enamel surface or any of the gingiva. The cleaning efficiency of toothpastes is directly related to their ability to retain active ingredients in the mouth. We have developed novel fluorescent methodology to evaluate the efficiency of toothpaste to retain in the oral cavity following brushing [10].
References:
1. Irmukhametova G.S., Mun G.A., Khutoryanskiy V.V., Langmuir, 27, 9551-9556 (2011)
2. Mun E.A., Williams A.C., Khutoryanskiy V.V., Int. J. Pharm., 512, 32–38 (2016)
3. Ways T.M.M., Lau W.M., Ng K.W., Khutoryanskiy V.V., Eur. J. Pharm. Sci., 122, 230-238 (2018)
4. Porfiryeva N.N., Semina I.I., Salakhov I.A., Moustafine R.I., Khutoryanskiy V.V., Nanomedicine: Nanotechnology, Biology, and Medicine 37, 102432 (2021)
5. Mun E.A., Morrison P.W.J., Williams A.C., Khutoryanskiy V.V., Mol. Pharm., 11, 3556-3564 (2014)
6. Mansfield E.D.H., de la Rosa V.R., Kowalczyk R.M., Grillo I., Hoogenboom R., Sillence K., Hole P., Williams A.C., Khutoryanskiy V.V., Biomat. Sci., 4, 1318-1327 (2016)
7. Al Mahrooqi J.H., Khutoryanskiy V.V., Williams A.C., Int. J. Pharm., 593, 120130 (2021)
8. Sevastos A.A., Thomson N.R., Lindsay C., Padia F., Khutoryanskiy V.V., Eur. Polym. J., 134, 109852 (2020)
9. Symonds B., Thomson N.R., Lindsay C.I., Khutoryanskiy V.V., ACS Appl. Mater. Interfaces, 8 (22), 14220–14230 (2016)
10. Aspinall S., Parker J., Khutoryanskiy V.V., submitted (2021)
Speaker
Vitaliy Khutoryanskiy
University of Reading
United Kingdom
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