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Morphological changes in rat lung tissue during inhalation of e-cigarette liquid aerosol

Alla B. Bucharskaya1,2,3, Dmitry A. Mudrak1, Irina Yu. Yanina2,3, Nikita A. Navolokin1,2, Alexey N. Bashkatov2,3, Elina A. Genina2,3, Yury V. Kistenev3, Valery V. Tuchin2,3
1 - Saratov State Medical University n.a. V.I. Razumovsky
2 - Saratov National Research State University n.a. N.G. Chernyshevsky
3 - Tomsk National Research State University


The morphological changes in the lung tissue of laboratory rats exposed to e-cigarette liquid ex vivo and in vivo were studied. For ex vivo studies the lungs of rats placed for 1 h in e-cigarette liquid. The in vivo study was performed on 12 outbred mature male rats weighing 200±20 g. The animals were divided into two groups, experimental and control, 6 rats in each group. The experimental group rats were placed in inhalation chamber and through a special installation, 1.5 litres of e-cigarette liquid aerosol was administered for 10 minutes, then a 2 minute - break, so - five times for 10 minutes - the rats received 7.5 litres of aerosol each. The rats of the control group were intact. The animals were removed from the experiment one hour after inhalation and their lungs were taken. Lung tissue samples were examined ex vivo by optical coherence tomography (OCT) and lung tissue spectroscopy. After manipulation, the lung samples were fixed in 10% formalin solution and embedded in paraffin. Then histological slices were made according to common methods with their subsequent staining with hematoxylin and eosin. The comparative analysis of ex vivo B-scan studies of lung tissue placed in e-cigarette liquid and control specimens demonstrate that the lung tissue is enlightened by the studied solution, as alveoli are filled with the solution and scattering is reduced. The structure of the tissue is different from that of the control samples. B-scan in vivo studies are characterised by the presence of voids of sufficiently large size. Histological examination of ex vivo and in vivo specimens showed morphological changes in lung tissue structure consistent with those observed in OCT.




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