Сombined usage of OCT-elastographyI and OCT-angiography for quantitative in vivo assessment of functional and morphological alterations in model tumors treated by PDT and chemotherapy
M.A. Sirotkina, Privolzhsky Research Medical University, Nizhny Novgorod
E.V. Gubarkova, Privolzhsky Research Medical University, Nizhny Novgorod
A.A. Plekhanov, Privolzhsky Research Medical University, Nizhny Novgorod
A.A. Sovetsky, Institute of Applied Physics RAS, Nizhny Novgorod
V.V. Elagin, Privolzhsky Research Medical University, Nizhny Novgorod
A.L. Matveyev, Institute of Applied Physics RAS,Nizhny Novgorod
L.A., Matveev, Institute of Applied Physics RAS, Nizhny Novgorod
S.S. Kuznetsov, N.A. Semashko Nizhny Novgorod Regional Clinical Hospital, Nizhny Novgorod
E.V. Zagaynova, Privolzhsky Research Medical University, Nizhny Novgorod
N.D. Gladkova, Privolzhsky Research Medical University, Nizhny Novgorod
V.Y. Zaitsev, Institute of Applied Physics RAS, Nizhny Novgorod
Abstract
Histological examination remains the gold standard in evaluation of tumor tissue state and its response to therapies, although preparation of histological images is invasive, laborious and rather time consuming procedure. By these reasons development of alternative methods which could at least partially eliminate some of the drawbacks of conventional histology has been attracting much attention. In this context various optical approaches are considered as attractive candidates to enable label-free diagnostics and in vivo utilization, including OCT and its functional extensions. In the reported study, to assess the tumor early reaction to therapies and predict its long-term response, for the first time we applied optical coherence angiography (OCA) in combination with another emerging OCT-extension - compressional optical coherence elastography (C-OCE). The developed OCE method opened unprecedented possibilities for monitoring of changes in the tumor morphology by in vivo performing tissue morphological segmentation with an accuracy comparable to that of conventional histology. Furthermore, OCA enabled the complementary characterization of tumor functional response. Such morphological and functional monitoring was demonstrated for two types of murine model tumors and different types of therapies – two kinds of chemotheraphy (antiangiogenic and cytotoxic) and vasculature-targeted photodynamic therapy. The results of OCE-based morphological segmentation showed high correlation with conventional histology. Such possibilities make OCE an attractive tool enabling previously inaccessible in vivo monitoring of individual tumor response to to therapies without taking multiple biopsies.
Speaker
V.Y. Zaitsev
Institute of Applied Physics RAS
Russia
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