In-vivo holographic laser speckle contrast imaging of brain tissues with pathological vascular conditions.
Arkady Abdurashitov - Skolkovo Institute of Science and Technology, Skolkovo Innovation Center, Building 3, Moscow, 143026, Russia.
Natalia Shushunova - Saratov State University, 83 Astrakhanskaya str., Saratov 410012, Russia.
Olga Sindeeva - Skolkovo Institute of Science and Technology, Skolkovo Innovation Center, Building 3, Moscow, 143026, Russia;
Saratov State University, 83 Astrakhanskaya str., Saratov 410012, Russia.
Oxana Semyachkina-Glushkovskaya - Saratov State University, 83 Astrakhanskaya str., Saratov 410012, Russia.
Valery Tuchin - Saratov State University, 83 Astrakhanskaya str., Saratov 410012, Russia.;
Tomsk State University, Tomsk, 634050, Russian Federation;
Institute of Precision Mechanics and Control, Russian Academy of Sciences, Saratov, 410028, Russian Federation.
Abstract
Laser speckle contrast imaging in a few recent years became one of the most common optical tools for functional imaging of different tissues. Incomplete theoretical description and sophisticated interpretation of measurement results are completely sidelined by a low-cost and simple hardware, fastness, consistent results, and repeatability. Relatively low probing depth for the visible spectral range and no depth selectivity are the two major drawbacks of the method. Furthermore, in a case of high NA objective lenses, the actual penetration depth of light in tissues is greater than depth of field (DOF) of an imaging system. Thus, the information about these out-of-focus regions persists in the recorded frames but cannot be retrieved due to intensity-only registration method. We propose a simple modification of LSCI system based on the digital holography principles to detect both amplitude and phase of scattering light, thus introducing the ability to after-registration refocusing, quantitative velocity measurements, vein-artery separation by the flow-direction and direct calculation of complex-amplitude autocorrelation function.
Speaker
Arkady Abdurashitov
Skolkovo Institute of Science and Technology
Russia
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