The influence of various anatomical and physiological parameters of the small intestine on its trans-serosal multimodal OCT imaging
Kiseleva Elena B., Privolzhsky Research Medical University, Institute of Experimental Oncology and Biomedical Technologies, Nizhniy Novgorod, Russia
Ryabkov Maxim G., Privolzhsky Research Medical University, University Clinic, Nizhniy Novgorod, Russia
Bederina Evgenia L., Privolzhsky Research Medical University, University Clinic, Nizhniy Novgorod, Russia
Sizov Mikhail A., Nizhny Novgorod State Budgetary Healthcare Institution “City Clinical Hospital No. 30”, Nizhniy Novgorod, Russia
Vorobyov Alexander N., Nizhny Novgorod State Budgetary Healthcare Institution “City Clinical Hospital No. 30”, Nizhniy Novgorod, Russia
Moiseev Alexander А., Institute of Applied Physics of the Russian Academy of Sciences, Nizhniy Novgorod, Russia
Gladkova Natalia D., Privolzhsky Research Medical University, Institute of Experimental Oncology and Biomedical Technologies, Nizhniy Novgorod, Russia
Abstract
Optical coherence tomography (OCT) is widely used in experimental studies to monitor various pathological conditions associated with structural and microcirculatory disorders in order to subsequently transfer the results into the clinic. The aim of the study was to evaluate the influence of anatomical and physiological parameters of intestinal wall on the sensitivity of multimodal optical coherence tomography (MM OCT) in diagnostics of the intestinal wall microstructure (with cross-polarization OCT mode) and microcirculation (with OCT angiography mode). During the course of experimental and clinical studies, the optical equivalents of micro-objects of the intestinal wall of rats, rabbits and patients who were operated on for acute mesenteric ischemia were compared.
MM OCT was performed with trans-serous access. Characteristics of the MM OCT setup were: radiation source - superluminescent diode, center wavelength 1310 nm, spectrum width 100 nm, scanning depth in air 1700 μm, scanning speed 20.000 A-scans / sec. Based on the analysis of 100 three-dimensional OCT images of rat intestines, 30 OCT images of rabbit intestines and 52 OCT images of human intestines, optical equivalents of subserous vessels with a diameter of 50-500 μm, thickness of smooth muscle layer and extravasal fluid accumulations were described and quantified.
It was shown that sensitivity of MM OCT in visualization of vessels of 50-100 microns directly correlates with the pulse rate and the number of erythrocytes (p = 0.014). MM OCT imaging of vessels with a diameter of 100-500 microns additionally correlated with the thickness ratio of the serous / muscular layers of the intestine. Sensitivity in visualization of outer longitudinal smooth muscle layer and extravasal fluid grew with an increasing the total thickness of the muscular membrane (p = 0.027).
Thus, the sensitivity of MM OCT in visualization of clinically significant objects of the intestinal wall microstructures is associated with hemodynamic parameters, concentration of red blood cells, and the thickness of the intestinal wall layers. The established patterns are necessary for a correct extrapolation of experimental data obtained on animals into the clinical MM OCT diagnostics of human intestinal diseases. The study was supported by the Russian Science Foundation, project No. 19-75-10096.
Speaker
Kiseleva Elena
Privolzhsky Research Medical University, Institute of Experimental Oncology and Biomedical Technologies, Nizhniy Novgorod
Russia
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