NUCLEIC ACID & AU NANOPARTICLE-BASED ADVANCED FUNCTIONAL BIOMATERIALS

Abstract

The recent applications of gold nanoparticles (AuNPs) in modern biomedicine include chemi- and biosensing; genomics and immunoassays; intracellular delivery of genes, drugs, peptides, and antigens; optical bioimaging and monitoring of cells and tissues; detection and photodynamic therapy of pathogenic microorganisms; detection and photothermolysis of cancer cells and tumors and wound repair [1]. The advantages AuNPs are related to their unique plasmonic properties; easy of fabrication with controllable shape, charge and sizes [2], and biofunctionalization with various ligands; high biocompatibility and low immunogenicity [3].
This talk is intended to summarize our recent efforts in analytical and theranostic applications of engineered AuNPs in conjunction with nucleic acid-based technologies [4]. Specifically, we provide examples of AuNP-based colorimetric [5, 6] and fluorescent [7] DNA detection systems, enhanced polymerase chain reaction [8], plasmonic-induced laser transfection system for intracellular gene delivery [9]. In addition to recently published reports, we discuss new concepts on AuNP conjugated with thiolated oligoDNA applied for fabrication of core-shell Au Raman tags with tunable sized interior gap; as alternative labels for Sousthern blotting; and as probes for in situ electron and dark-field microscopy of targeted nucleic acids in cells and tissues. For each of the above topics, the basic principles, recent advances, and current challenges are discussed.
This work was supported by the Russian Science Foundation (synthesis and characterization of nanoparticles in the framework of project No. 18-14-00016, cell experiments in the framework of project No. 17-74-10090).

References.
1. Lev Dykman, Nikolai Khlebtsov. Gold Nanoparticles in Biomedical Applications // CRC Press, Boca Raton and London, 2017. ISBN 978-1-138-56074-1.-322 pp.
2. Nikolai G. Khlebtsov, Li Lin, Boris N. Khlebtsov, Jian Ye. Gap-enhanced Raman tags: fabrication, optical properties, and theranostic applications. Theranostics 10 (5), 2067-2094 (2020).
3. Dykman L.A., Khlebtsov N.G. Gold nanoparticles in chemo-, immuno-, and combined therapy: review [Invited]. Biomed. Opt. Express 10 (7), 3152-3182 (2019).
4. Wang, Z.-Y., Li, P., Cui, L., Qiu, J.-G., Jiang, B., Zhang, C.-Y. Integration of nanomaterials with nucleic acid amplification approaches for biosensing (2020) TrAC - Trends in Analytical Chemistry, 129, art № 115959
5. Pylaev T., Khanadeev V., Khlebtsov B., Dykman L., Bogatyrev V., Khlebtsov N. Colorimetric and dynamic light scattering detection of DNA sequences by using positively charged gold nanorods and nanospheres: A comparative study. Nanotechnology, 22, 285501 2011.
6. Khlebtsov B. N., Pylaev T. E., Khanadeev V. A., Khlebtsov N. G. Application of Dynamic Light Scattering and Absorption Spectroscopy to Studies of Systems with Colloidal Gold Nanoparticles + DNA. Izv. Saratov Univ. (N.S.), Ser. Physics, 2017, vol. 17, iss. 3, pp. 136–149 (in Russian).
7. Pylaev T. E., Volkova E. K., Kochubey V.I., Bogatyrev V.A., Khlebtsov N. G. DNA detection assay based on fluorescence quenching of Rhodamine B by gold nanoparticles: the optical mechanisms. J. Quant. Spectrosc. Radiat. Transfer, 131, 34-42, 2013
8. Vanzha E.V., Pylaev T.E., Khanadeev V.A., Konnova S.S., Fedorova V.A., Khlebtsov N.G. Gold nanoparticle-assisted polymerase chain reaction: effects of surface ligands, nanoparticle shape and material // RSC Adv. 2016. V. 6. P. 110146 - 110154
9. Pylaev T., Vanzha E., Avdeeva E., Khlebtsov B., Khlebtsov N. A novel cell transfection platform based on laser optoporation mediated by Au nanostar layers. J. Biophotonics12. art. e201800166 (2019).

Speaker

Timofey E. Pylaev
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences,Saratov State Medical University n.a. V.I.Razumosky, Agricultural Research Institute of South-East Region
Russia

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