Assessment of changes in the viscosity properties of tumor cell membranes under the influence of chemotherapeutic agents
Liubov Shimolina1, 2, Marina Shirmanova1, Marina Kuimova3, Maria. Lukina1, Irina Druzhkova1, Elena Zagaynova1
1 Privolzhsky Research Medical University, Nizhny Novgorod, Russia
2 Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod, Russia
3 Imperial College London, South Kensington, London, United Kingdom
Abstract
Membrane viscosity is one of the most important properties characterizing a living cells. Changing the viscosity is reflected on the physical and biochemical processes essential for the cell. Chemotherapy is widely used to treat various forms of cancer, especially cisplatin. However, the platinum drugs action at the cellular level still remains unknown. The aim of our research was to study plasma membrane microviscosity of cancer cells using fluorescent molecular rotor BODIPY 2 and fluorescence lifetime imaging FLIM during chemotherapy. The study was performed on cultured cancer cells CT26 (mouse colorectal cancer) and Hela Kyoto (human cervical cancer) and on mice Balb/c with CT26 tumors. Multiphoton tomograph MPTflex (JenLab, Germany) equipped with a TCSPC-based FLIM module (Becker&Hickl Inc., Germany) was used to detect the fluorescence lifetime of a molecular rotor. Using FLIM we investigated the changes of microviscosity of plasma membrame in cancer cells in vitro exposed to chemotherapy. Significant increase of viscosity was detected in 24 h after cisplatin treatment. A protocol for measuring microviscosity in mouse model tumors in vivo was also developed. The study of the action of drugs on the viscosity of living cells is important for a deep understanding of the mechanisms of their cytotoxicity. «This work is supported by the Russian Science Foundation under grant No: 20-14-00111.
Speaker
Liubov Shimolina
Privolzhsky Research Medical University
Russia
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